Grease a 9"x5" loaf pan with cooking spray and line with parchment paper. Reheat oven to 375˚F
In a small bowl combine 2½ cups all purpose flour, 1 teaspoon baking powder, and ½ teaspoon baking soda.
Zest 2 lemons, and squeeze the juice out of both. Set aside 1 Tablespoon of the juice for the glaze.
In a small bowl mix 1 cup sour milk with ½ cup lemon juice and 2 Tablespoon lemon zest.
In a medium mixing bowl, blend softened ½ cup salted butter and 1 cup sugar together well. Add 2 large eggs and whisk well. Add 1 Tablespoon poppyseeds.
Mix the flour and sour milk mixture alternately into the egg mixture, folding between each addition, until just combined.
Fold into prepared pan and bake for 15 minutes at 375˚F. Turn oven down to 350˚F and continue to bake for 45 minutes.
Check for doneness by a toothpick inserted into the middle of loaf comes out clean. Cool on a wire rack.